Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
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چکیده
منابع مشابه
Thermal and spectroscopic characterization of a proton pumping rhodopsin from an extreme thermophile.
So far retinylidene proteins (∼rhodopsin) have not been discovered in thermophilic organisms. In this study we investigated and characterized a microbial rhodopsin derived from the extreme thermophilic bacterium Thermus thermophilus, which lives in a hot spring at around 75 °C. The gene for the retinylidene protein, named thermophilic rhodopsin (TR), was chemically synthesized with codon optimi...
متن کاملActive state of sensory rhodopsin II: structural determinants for signal transfer and proton pumping.
The molecular mechanism of transmembrane signal transduction is still a pertinent question in cellular biology. Generally, a receptor can transfer an external signal via its cytoplasmic surface, as found for G-protein-coupled receptors such as rhodopsin, or via the membrane domain, such as that in sensory rhodopsin II (SRII) in complex with its transducer, HtrII. In the absence of HtrII, SRII f...
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Light-driven ion-pumping rhodopsins are widely distributed in microorganisms and are now classified into the categories of outward H(+) and Na(+) pumps and an inward Cl(-) pump. These different types share a common protein architecture and utilize the photoisomerization of the same chromophore, retinal, to evoke photoreactions. Despite these similarities, successful pump-to-pump conversion had ...
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G-protein-coupled receptors (GPCRs) transmit stimuli to intracellular signaling systems. Rhodopsin (Rh), which is a prototypical GPCR, possesses an 11-cis retinal. Photoisomerization of 11-cis to all-trans leads to structural changes in the protein of cytoplasmic loops, activating G-protein. Microbial rhodopsins are similar heptahelical membrane proteins that function as bacterial sensors, ligh...
متن کاملProton Pumping P Systems
We propose here a (biologically inspired) model of P system called proton pumping P system that is a special case of evolution– communication P system. In cell biology there are transport mechanisms, involving protons. We generalize this idea by considering a few different types of protons. A proton pumping P system is, essentially, an evolution–communication P system where a special subset of ...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-58023-5